Biosafety Training Information

1. Collaborative Institutional Training Initiative (CITI) online training program

Effective March 1, 2015, any person involved with the use, handling or storage of biohazards is required by the Institutional Biosafety Committee (IBC) to complete a special course in Biosafety and Biosecurity (BSS).  A refresher course must then be completed every 5 (five) years to stay in compliance with the new IBC requirement. Additionally, any person or student that works in any biosafety level (BSL) laboratory must also complete the course Biosafety and Biosecurity online, regardless of the project assigned.

The course is available through the University’s subscription to the online training program and is designed to provide a comprehensive, conceptual framework for the responsible and safe use of biohazards in research and teaching. Click here for CITI online training program.

For any CITI training, create an account on the website and follow the instructions below. 

Briefly, an online survey consisting of several required and optional questions will populate your learner account and assign you to the appropriate Learner Group.  The answers to question #8 will automatically populate your individualized Biosafety related course curriculum. 

New to CITI?  Download instructions for initial set up of your CITI account.

Already have a CITI account?  Download instructions to add the Biosafety & Biosecurity Course.

Need to Affiliate with UNC Charlotte’s CITI Account?  Download instructions on Affiliating with Another Institution.

Online Biosafety required courses available at CITI and the groups that must take it:

  • Laboratory Personnel BSS: Researchers, students, special volunteers, and any person that works in a BSL-1, BSL-2 or BSL-3 laboratory.
  • Principal Investigators BSS: Director/Head/Principal Investigators planning to use or handle biohazards.
  • NIH Guidelines for Research Involving Recombinant  and Synthetic Nucleic Acid Molecules: Any lab member that works with or handles recombinant or synthetic nucleic acids.
  • Shipping and Transport of Regulated Biological Materials: Any lab member or staff that prepares the material, the package or the paperwork for shipping dangerous goods materials (including laboratory samples and dry ice). Note: Training certificate is valid for 2-years.
  • Animal Biosafety: Researchers, Principal Investigators, students, special volunteers, and any person that injects infectious microorganisms into animals.
  • USDA Permits: Researchers, Principal Investigators, students, special volunteers, and any person that works with material regulated by USDA.
  • Institutional Biosafety Committee (IBC) Members BSS: IBC members (optional course).

2. Bloodborne Pathogens (BBP) Training on Skillport

Bloodborne pathogens are infectious microorganisms present in blood that can cause disease in humans. These pathogens include, but are not limited to, hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). Workers exposed to bloodborne pathogens are at risk for serious or life-threatening illnesses 

At UNC Charlotte every faculty, student, staff or volunteer that works with: Blood, blood by-products, unfixed tissues, body fluids/secretions, primary or established cell lines from human or non-human primates, must enroll in the UNC Charlotte’s Bloodborne Pathogens Exposure Control Plan managed by EHS (704-687-1111). Part of the BBP Exposure Control Plan involves an online course, the "Bloodborne Pathogen Awareness" available through a separate online training system called Skillport. Click here for the Skillport training program. The BBP Awareness training must be retaken every year before the anniversary of the initial completion date. This requirement is in accordance with the Occupational Safety and Health Administration (OSHA) Bloodborne Pathogen Standard (29 CFR 1910.1030). To complete the BBP Awareness training in Skillport, please follow the instructions below.

•    Bloodborne Pathogen Awareness (ID: esh_sah_b19_sh_enus)

a)    Go to
b)    Click on Login
c)    Log in with your UNCC credentials
Note: If you have any problems logging in on Skillport, please contact IT Help Desk (704-687-5500) and ask to be added to Skillport system
d)    Search for esh_sah_b19_sh_enus
e)    Click on Bloodborne Pathogens Awareness course
f)    Click Launch to complete the course
Note: You can log in and log out and complete the entire course over multiple sessions if needed

3. Autoclave Use

The link provided here provides an overview of autoclave use for initial or refresher training for all lab personnel who are expected to work with autoclaves. 

Source: David Gillum, Arizona State University, YouTube video published November 6, 2015.

NOTE: The video advises that clear OR red biohazard bags can be used for autoclaving for ultimate disposal in regular trash. However, the video was made at an institution located in another state. Please consult your PI, the Biosafety Officer at 704-687-1825 or posted signage to follow appropriate autoclaving techniques which are in line with NC regulated waste disposal laws.

4. Proper Use of a Biosafety Cabinet

Click here for a video series on the proper use of a Biosafety Cabinet developed by NuAire (biosafety cabinet manufacturer) available for.

Need more information? Contact:

Dr. Angelica Martins, Biosafety Officer, 704-687-1825